E-Mail : info@incozen.com

Pharmacology Services

Functional Pharmacology

INCOZEN offers an array of In Vitro Pharmacology services for drug discovery and early lead identification, customized primary and secondary assays to support the mechanistic aspects including elucidation of signaling cascades across several therapeutic areas.

Specific Enzyme Assays for selected targets (End Point and Kinetic Mode)
  • Luminometry
  • Fluorometry (including Time resolved)
  • Absorbance
Customized Cellular Assays in immortalized or primary cells relevant to the target of interest
  • Functional activity of cells upon antigen induction (calcium influx, cytokine release, inflammatory mediators such as elastase, histamine, superoxides etc.)
  • Basophil Activation Test by Flow Cytometry
  • Cytotoxicity
  • Down-stream biomarker assays
  • Reporter Gene Assays for targets
  • Plasmid Cloning and Transfection for generation of Stable and Transient Transfectants
Cellular Oncology

Within oncology, INCOZEN offers a wide array of assays using several adherent, semi-adherent, or suspension tumor cell lines. The assays listed below can be customized based on the sponsor’s request.

  • Cell Proliferation Studies (MTT or Alamar Blue)
  • Apoptosis (Fluorescence assay or Flow Cytometry)
  • Lymphocyte (T & B cells) proliferation using specific tagged antibodies (Flow cytometry)
  • Cell cycle analysis by Flow Cytometry
  • Tumor cell invasion Assays
  • Tumor cell migration Assays
  • Tumor cell scatter Assays
  • Trans-endothelial migration Assays
  • Down Stream Biomarker estimation (by ELISA or Western Blot)
  • Immune cell depletion assay
  • Antigen induced cytokine estimations
  • Cytotoxicity in cell lines
In Vivo Efficacy Models

INCOZEN offers an array of In Vitro Pharmacology services for drug discovery and early lead identification, customized primary and secondary assays to support the mechanistic aspects including elucidation of signaling cascades across several therapeutic areas.

Inflammation Disease Models
  • Concanavalin A induced hepatitis in mice
  • Concanavalin A induced cytokine release in mice
  • TNCB induced ear CHS in mice
  • Oxazolone induced ear delayed type hypersensitivity in mice
  • Methylated Bovine serum albumin induced footpad DTH in mice
  • DNFB induced ear DTH in mice
  • LPS induced neutrophil migration in a rat air pouch model
  • LPS induced pulmonary neutrophilia in mice/rat
  • Carrageenan induced paw oedema in mice/rat
  • LPS induced TNFα in mouse/rat
  • Anti-IgE induced histamine release in mice
  • Passive cutaneous anaphylaxis in mice
  • Endotoxin induced uveitis in rat
  • Methionine & choline deficient diet induced non-alcoholic steatohepatitis in mice
  • Anti-IgE induced histamine release in mice
Arthritis
  • Collagen induced arthritis in female Wistar rat
  • Adjuvant induced arthritis in Wistar rat
Colitis
  • DNBS induced colitis in Wistar rat
Diabetes
  • Streptozotocin –nicotinamide induced insulin resistance
  • High fat Diet induced obesity in mice
  • HFD & STZ induced insulin resistance
Asthma/COPD
  • Acute/chronic Cigarette smoke induced COPD in mice
  • Acute/chronic Cigarette smoke extract induced COPD in mice
  • Ovalbumin induced asthma in guinea pig
  • Ovalbumin induced mast cell degranulation in Guinea Pig
  • Ovalbumin induced asthma in mice
  • DNFB sensitized DNBS induced non-atopic asthma in mice
  • PAF induced pulmonary eosinophilia in Guinea Pig